Live Vaccine Against Newcastle Disease, Based on the LaSota Strain

The live Newcastle disease vaccine is used to prevent Newcastle disease and induce effective immunity in poultry flocks. Determining the appropriate vaccination schedule based on maternal antibody levels and serological test results, together with proper administration and adherence to recommended storage conditions, plays an important role in improving vaccine efficacy and maintaining flock health.

Technical Specifications

Vaccine Trade Name:	ARRIAH-LaSota
Dosage Form:	Lyophilized powder for reconstitution
Vaccine Composition:	The vaccine contains live attenuated Newcastle disease virus (NDV) LaSota strain propagated in specific-pathogen-free (SPF) embryonated chicken eggs and harvested from the allantoic fluid.
Antigen Content:	Each immunizing dose contains 7.0 log₁₀ EID₅₀ of Newcastle disease virus (LaSota strain).
Vaccine Presentation:	Vial containing 4000 doses
Immunogenicity:	The vaccine induces an immune response against Newcastle disease virus within 6–8 days following a single administration. The resulting immunity persists for approximately 1–2 months.
Target Species & Indications:	Intended for active immunization of broiler chickens laying hens and breeding flocks against Newcastle disease.
Routes of Administration:	Administration via drinking water ocular instillation (eye drops) or spray vaccination.

Product Introduction

Adverse Effects

When the vaccine is administered by spray in accordance with the instructions, chicks may show signs of illness, dyspnea, and reduced appetite on days 4 or 5 after vaccination. These symptoms usually resolve spontaneously.

Evaluation of Immunity

The optimal vaccination schedule is determined based on hemagglutination inhibition (HI) titers or maternal antibody levels measured by ELISA in 10-day-old chicks. At least 25 serum samples should be collected from chicks housed in the same poultry house for testing.

ELISA and HI Testing Criteria:

Birds should be vaccinated when the Newcastle disease virus HI antibody titer is below 1:8 in 20% of the tested serum samples and the ELISA antibody titer is below twice the positive threshold specified in the diagnostic kit instructions.

If more than 80% of serum samples show an HI antibody titer of 1:8 and ELISA titers are at least twice the positive threshold specified in the diagnostic kit instructions, the birds should be tested regularly every 3–5 days. Vaccination should be performed when the level of immunity decreases to 80%, meaning that at least 20% of serum samples show an HI antibody titer of 3 log₂ or higher (equivalent to 1:8).

Precautions for Use

Chicks must not be vaccinated later than 3 weeks before the onset of egg production.

Precautions for Vaccinators

All personnel involved in vaccination must wear protective clothing, such as coveralls or a coat, boots, and a cap or other head covering. Appropriate personal protective equipment, including safety goggles and a suitable mask, must also be worn. First-aid kits must be available at the workplace.

In the event of accidental contact between the vaccine and the skin or mucous membranes, the affected area must be rinsed thoroughly with plenty of water. After handling the vaccine, hands must be washed thoroughly with soap and water.

In the event of a vaccine spill, the contaminated area must be disinfected with either a 5% Chloramine B solution or a 2% sodium hydroxide solution.